The human capital is unarguably an important element that completes any organization. With proper motivation, these people work towards quality products and services. Question arises on non-teaching personnel of academic institutions as support staff. This study focuses on the work motivations and job performance of non-teaching personnel in a university. This descriptive research used structured questionnaire for the self-assessment of the 50 administrative and support staff. The results of the study proved that the workplace environment and professional growth and development are considered highly influential motivational factors. Furthermore, there is a positive significant relationship between the motivational factors and job performance as to quantity and quality of work. It also confirmed that professional growth and development has significant relations with the attitude towards work. The results of this study can be an input to the University Human Resource planning. Since the study is focused only on a single university, a comparative study with another higher education institution is recommended.